Reporter

Part:BBa_K1486049:Design

Designed by: EPFL iGem team 2014   Group: iGEM14_EPF_Lausanne   (2014-10-07)


CpxR promoter FW + RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 727
    Illegal AgeI site found at 839
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The regulatory sequence is the native CpxR responsive site found between the coding sequences for CpxA and CpxP in the E.coli genome in the direction the CpxA is coded. The RFP has a start and a stop codon.

Source

The basis of this part is the BBa_K339007 which had just the RFP sequence. We inserted the non coding sequence found in the the E.coli genome.

References